Expression Analysis Services

From Genome Technology Core (GTC) wiki - Sequencing and Microarray

Expression Analysis - Microarray Expression Analysis - Microarray

We offer a Expression Analysis Service utilizing the 3 Plaforms.

Key highlights of the service:

Please see below for further details about the service.

Contents 1 Sample Submission Guidelines 2 mRNA Detection Platform 3 Protocol 4 Data Extraction 5 Analysis 6 Pricing 6.1 Affymetrix 6.2 Agilent

Sample Submission Guidelines

• Customer should submit atleast 5 ug of Total RNA or 2 ug of Samples enriched for miRNA.
• RNA concentration and quality should be quantified.
• Sample submission forms should be submitted along or before the samples are submitted. The form is available here.

mRNA Detection Platform

• Affymetrix.
• Agilent

Protocol

Data Extraction

• Microarray images are scanned for a balanced view and saved in TIFF formats.
• Numerical intensities are extracted for each probes
• The results are organized which includes TIFF image files and intensity data files, which is made available to the customer in a CD.

Analysis

• We would do the image analysis and provide you with the raw intensities for all the platforms.
• Certain downstream data processing is done based on the platform, as part of the service:
  • Affymetrix data is scaled to a average intensity of 150 per chip.
  • Agilent and other 2 color channel data is normalized within array.

Additional data analysis help may be available on a case by case basis.

Pricing

Prices for sample processing do not include cost of arrays

Affymetrix

Item	Unit	Price Per Unit	Notes
Affymetrix GeneChip Expression Array	Array	~$400	Array only
Standard Expression Processing	Sample	~$500	>5ug total RNA
Small Sample Expression Processing	Sample	~$650	10-100ng total RNA
Prokaryotic Protocol	Sample	~$450	-

Agilent

Item	Unit	Price Per Unit	Notes
Agilent Expression Array 4x44k multiplex	"Slide (array)"	~$700/slide	($175/array)
Standard Expression Processing	Sample	~$350	Per sample, single channel
ChIP-on-Chip Processing	Sample	~$650	Two samples, dual channel