HCSCellomics

From Genome Technology Core (GTC) wiki - Sequencing and Microarray

The GTC features the complete system for High Content Screening (HCS) to drug discovery and systems biology researchers using the Thermo Scientific Cellomics Machine. The system offers a total platform, comprising automated imaging instrumentation, BioApplication image analysis software and high content informatics.

Information about Instrumentation
Information about Bioapplications Please note: We currently have licenses for Compartmental Analysis, Morphology Explorer, Spot Detector and Target Activation.
Information about Software Platform

Accessing Data

You can either access your data from the Instrument computer or here: http://array.wi.mit.edu/hcsexplorer/

Access and Availability

Access to the HCE Cellomics is available via a Zimbra calendar system. Sign-up is required. It is recommended that you sign up at least 48 hours in advance to ensure availability.

To View Calendar Access: https://mars.wi.mit.edu/home/hcs-cellomics/Calendar.html [LOGIN USING WI USERNAME AND PASSWORD].

To book a slot on the calendar (External customers, please contact us directly): You can use the same system as the FACS machine i.e. using the zimbra web interface. Instructions on reserving HCS cellomics machine using Zimbra:

1. Login to the Zimbra web client, https://mars.wi.mit.edu

2. Select the calendar tab and then click New, this will take you to an appointment screen. If you only see a “Quick Add Appointment” dialog box, click the “more details …” button in the bottom left of the box.

3. The Subject should contain your Name, Lab and phone number. Set the start and end times according to your needs.

4. Add the equipment by clicking on the “Find Resources” tab. You can type the name “hcs-cellomics” and then the Search button, if you don’t know the name the Search button alone will list all Resources. Verify the equipment is available during your meeting time by looking in the status column. A BUSY means the equipment is already reserved at that time, you will need to find a different time for your meeting. If the equipment is Free, select it and click add, this will invite the equipment to the meeting.

5. Save the meeting! You will receive an email verification of the appointment. The meeting will now be on your own calendar and the equipment calendar. The equipment will respond immediately with confirmation if it’s available.

6. If your meeting does not update on the calendar, click the refresh button on the toolbar directly above your calendar.

New users should contact Sumeet Gupta [sgupta at wi dot mit dot edu] to schedule an orientation session.

Sign-up policy

• Fill out the your name, PI and phone number when booking a time slot on the calendar.
• To cancel, please do so 24 hours in advance so that others can use the slot.