SubmissionGuide

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Please be advised that WIGTC cannot archive customer samples (input materials and libraries) for longer than three months from the time of submission. Please contact us if you would like to arrange to collect your samples during that time.

Contents

SEQUENCING SUBMISSION GUIDELINES

You can download detailed guidelines in word format from here.

STEP 1: REGISTRATION (FOR NEW CUSTOMERS ONLY)

  • INTERNAL CUSTOMERS: Please click here to register in our system.
  • EXTERNAL CUSTOMERS: Fill out a new customer form (Available Here) with billing information. Once the form is emailed to us, we will register you in our database and notify you when you can submit samples to us.

All external customers please obtain a purchase order number prior to your submission. If you need a quote, please email us at wibr-genome@wi.mit.edu or call us at 617-258-8803. Blanket purchase orders are preferred, if you plan to submit multiple projects over time.


STEP 2: PREPARATION OF SAMPLES

SUBMISSION OF SAMPLES FOR LIBRARY PREPARATION

Samples should be in a buffer that does not contain trace amounts of phenol or EDTA. We will save and return any unused input material after libraries are successfully prepared.

Below are our input requirements for Library Prep Service

Sample Type Sonication/Fragmentation* Fragment Size (bp) Input requirements (ng)** Volume (ul)
Genomic Required 200 to 400 500 to 1000 10-50
ChIP Required Variable (specify) 10 to 50 10-40
RNA (total) NA NA 100 to 1000 10-50

*Unless special arrangements are made with us in advance, we will assume that your genomic DNA sample has been fragmented, by sonication or otherwise, prior to submission.

**We can work with less if necessary, but it is best if you elute your DNA in 50 ul or less if you believe we may need to use all of it. Please give us at least 10uL of sample to ensure we have enough for QC and prep.

SUBMISSION OF PRE-PREPARED LIBRARIES

We typically need 10-20 ul of each library at 5 ng/ul or greater. If your library is less concentrated than 5 ng/ul, we’ll quantify it to make sure we have enough to sequence.


STEP 3: ONLINE SUBMISSION OF SEQUENCING LIBRARIES

  • Please complete the online submission form (Link To Form Available Here). Questions or problems related to the submission should be directed to Sumeet Gupta at sgupta@wi.mit.edu
  • Every physical sample tube should have its own Genome Tech Core ID (GTC ID) number (automatically generated and emailed to you immediately after submission of the online form), even if you plan to multiplex them.
  • Please write your name GTC ID numbers on the tubes. We prefer 1.5 ml tubes.


STEP 4: DROP-OFF OR SHIPPING

When you are ready, you may hand deliver or ship samples them on dry ice to:

Whitehead Institute Genome Technology Core
9 Cambridge Center, Room 325
Cambridge, MA 02142

Please do not ship without first contacting someone in the lab, to make sure that we are here to receive your package. Do not rely on voicemail. Please wait until you hear back from someone.


MICROARRAY SUBMISSION GUIDE

You can download a word document of the guidelines here.

STEP 1: PAPERWORK/PREPARATION FOR MICROARRAY SUBMISSIONS (EXTERNAL CUSTOMERS ONLY)

  • Please contact us in advance of submitting any microarray projects, for approval. Wibr-genome@wi.mit.edu
  • New external customers, please fill out a new customer form (Available Here) with billing information. Email the completed form to wibr-genome@wi.mit.edu.

All external customers, please contact your Accounts Payable department in advance of submission, to obtain a purchase order number. If you need a quote, please email us at wibr-genome@wi.mit.edu or call us at 617-258-8803. Blanket purchase orders are preferred, if you plan to submit multiple projects over time.


STEP 2: PREPARATION OF SAMPLES FOR MICROARRAY SUBMISSION

We typically use 100 ng – 1 ug of total RNA in 3 ul as input for microarray sample preparation, so your RNA should be at least 33 ng/ul. We can work with less if necessary, but it is best if you elute your RNA in as little volume as is practical to avoid having to dry down. Any buffer that does not contain trace amounts of phenol or EDTA is acceptable. We will save and return any unused input material and microarray-ready samples to you after the arrays have been done.


STEP 3: FILL OUT AND EMAIL THE SUBMISSION FORM

Microarray submission form is available here. Please fill out and email the microarray submission form to wibr-genome@wi.mit.edu. Please note that due to contractual obligations, we are not able to purchase Affymetrix arrays for customers outside of the WI/MIT community. External customers should order arrays and ship them along with samples. Affymetrix may also be able to ship your order to us directly.


STEP 4: DROP-OFF OR SHIPPING

You may drop off samples to us in person in room 325, or ship them on dry ice (see below). Please do not ship to us without first contacting someone in the lab, to make sure that we are here to receive your package. Please email wibr-genome@wi.mit.edu or call 617-258-8803 prior to shipping. Do not rely on voicemail. Please wait until you hear back from someone.

When you are ready, hand deliver or ship your samples to:
Whitehead Institute Genome Technology Core
9 Cambridge Center, Room 325
Cambridge, MA 02142

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