Zeiss AxioPlan 2

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Hardware:

Filters:

Not sure which fluorophores will work best? Use an online spectra viewer (see Links) or contact Wendy.

Software setting Excitation Dichroic Emission Source
DAPI G 365   445/50M Zeiss (filter 49)
GFP HQ470/40X   HQ525/50M Chroma
TxRed 565/40X FT585 620/60x Zeiss (filter 31)
Cy5 628/40X   692/40M Chroma
CFP D436/20x   D480/40m Chroma
YFP D500/20x   D535/30m Chroma
DIC Tungsten Halogen   Analyzer Zeiss
Trans Tungsten Halogen   --  

All numbers in nanometers.

Objectives:

Mag. NA

Immersion Medium

Phase contrast DIC prism Corrections HamamatsuScaling** (um/pixel)
5x 0.25 Air -- -- Fluar  
10x 0.30 Air -- I EC Plan NeoFluar 0.645
20x 0.5 Air Ph1 II Plan NeoFluar 0.317
40x 0.75 Air Ph2 II Plan Fluor 0.162
*40x 1.3 Oil -- II Plan NeoFluar 0.163
63x 1.4 Oil -- III Plan Apochromat 0.102
100x 1.4 Oil Ph3 III Plan Apochromat 0.065
*100x 1.4 Oil -- III Plan Apochromat 0.065

*Objectives marked with an astrisk are not permanently installed. Please contact Wendy for installation.
** These values work well for making scale bars. When performing careful distance measurements, it is best practice to confirm the calibration for the exact setup you use.

Sample holders:

Standard 3 x 1in slides with #1.5 glass coverslip

Software:

To view files in lab, many users like to use Fiji (ImageJ) with the BioFormats plugin for importing the .nd file. See our Links page to download.

 

 
 

 

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