Zeiss LSM 700 confocal

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Hardware:

Filters:

Not sure which fluorophores will work best? Check out an online spectra viewer (see Links) or contact Wendy.

Widefield filter sets for viewing samples by eye:
  • DAPI
  • FITC/GFP
  • Rhodamine
Confocal emission filters, used in conjunction with the variable secondary dichroic (VSD) beamsplitter
Detector 1 Detector 2

490-555nm

505-600nm
490-635nm LP 490nm
SP 490nm LP 560nm
SP 555nm LP 615nm
SP 640nm LP 640nm
465-505nm + LP 525nm 555-610nm + LP 650nm
420-470nm + 500-610nm 495-535nm + LP 555nm

Objectives:

Mag. NA

Immersion Medium

DIC Correction Working
Distance (mm)
5X 0.13   NA    
10X 0.45 Air NA Plan NeoFluar 2.0
20X 0.8 Air II Plan Apochromat 0.55
25X 0.8 Water, Glycerol, or Oil III LCI Plan NeoFluar Corr 0.21
40x 1.3 Oil NA Plan Apochromat 0.21
63X 1.4 Oil II Plan Apochromat 0.19

Software:

To view your .lsm files in lab, see our Links page for Zen lite 2011 (requires Windows operating system) or ImageJ.

 

 

 
 

 

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