Zeiss LSM 710
Laser Scanning Confocal

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Hardware:

Filters:

Not sure which fluorophores will work best? Check out an online spectra viewer (see Links) or contact Wendy.

Widefield (viewing samples by eye):

  • DAPI
  • FITC/GFP/Alexa 488
  • Rhodamine/ Alexa 568
  • DIC

Confocal emission is spread into a spectrum with a diffraciton grating. Any range of wavelengths 371-740nm can be sent to one of three PMT detectors. Note that PMT 3 has higher sensitivity for red wavelengths than the other two detectors. For specific information on thel asers and detection wavelengths used for an image, look in the metadata of your .lsm or .czi file.

Objectives:

Mag. NA

Immersion Medium

DIC Corrections Working
Distance (mm)
2 photon optimized?
10X 0.30 Air -- Plan NeoFluar 5.5  
25X 0.8 corr Water, Glycerol, or Oil -- LD LCI Plan Apochromat 0.57 YES
40X 1.1 corr Water III LD C-Plan Apochromat 0.62 YES
40x 1.3 Oil III Plan Apochromat 0.21  
63X 1.4 Oil III Plan Apochromat 0.19  

corr = objective has a correction collar that must be adjusted to prevent spherical aberration.
Spatial scaling is dependent on the user-defined frame size and zoom. See your.lsm or .czi file.

Sample holders:

All objectives expect a #1.5 glass coverslip.

Stage plates Compatible Sample holders
Adjustable Slides, LabTek II chambers, 35mm dishes, 60mm dishes
Multipwell plate Any standard 96-well plate format

Software:

To view your .lsm files in lab, see our Links page for Zen lite 2011 (requires Windows operating system) or the Fiji package of ImageJ.

 

 

 
 

 

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