Zeiss Lightsheet Z.1

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Hardware:

  • Zeiss Lightsheet Z.1 light sheet fluorescence microscope
  • 2 PCO.edge sCMOS cameras
  • Solid state excitation lasers:
    405nm, 440nm, 491nm, 514nm, 561nm, and 640nm
  • Transmitted light
  • Zeiss Zen acquisition software
  • 32TB temporary file storage

References:

Filters:

Not sure which fluorophores will work best? Check out an online spectra viewer (see Links) or contact Wendy.

Pos Menu name Fluorophores Camera 1 Filter Camera 2 Filter
1 mirror -- -- --
2 LP490 DAPI + GFP 420-470nm 505-545nm
3 LP510 CFP + YFP 460-500nm 525-565nm
4 LP560 GFP + mCherry 505-545nm LP 585nm
5 LP560 DAPI & GFP +
mCherry & DRAQ5
425-460nm &
505-545nm
570-610nm &
670-735nm
6 LP640 mCherry + DRAQ5 575-615nm LP 660nm

Objectives:

Mag. NA

Immersion Medium

RI
5X 0.15 Air 1.33 or 1.45
20X 1.0 Water dipping 1.33
40X 1.0 Water dipping 1.33
20X 1.0 CLARITY dipping 1.45

Software:

  • Zeiss Zen - basic post processing, data handling, manual 3D multiview reconstruction
  • arivis vision4D - visualization of very large datasets
  • Fiji - post processing, data handling, automated 3D multiview reconstruction

 

 

 
 

 

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