Questions on which microscope is best suited for your experiments? Contact Nicki Watson or Wendy Salmon

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EQUIPMENT

1. Zeiss LSM 700 Laser Scanning Confocal
-Excitation lasers: 405, 488, 555, 639nm

2. Zeiss LSM 710 NLO
-3 spectral channel (filter free) laser scanning confocal
-2 Non-descanned detectors with blue, green, and red filters for 2-photon detection
-Excitation lasers: 405, 458, 488, 514, 561, 594, 633nm + Coherent Chameleon Ultra II femtosecond pulsed-IR tunable from 690-1064nm

3. PerkinElmer Ultraview Spinning Disk Confocal
-Volocity acquisition software and Hammamatsu ORCA-ER CCD camera 
-Excitation lasers: 405, 440, 488, 514, 568, 640nm

4. Andor Spinning Disk/TIRF system
-Andor IQ acquisition software and Andor iXion+ EMCCD cameras. 
-Additionally equipped with Andor Revolution FRAPPA device and LiveCell Stagetop incubation system. 
-Excitation lasers: 405, 488, 561

5. Nikon TE2000 automated inverted microscope with incubation enclosure
-MetaMorph acquision software and Hammamatsu Orca-ER camera. 

6. Zeiss AxioPlan2 upright microscope
-AxioVision acquisition software and Hammamatsu Orca-ER camera
- Qimaging Color Micropublisher 3.3 CCD camera with QCapture software

7. FEI Technai Spirit Transmission Electron Microscope

8. Jeol 5600LV Scanning Electron Microscope

9. Reichert Ultracut E Ultramicrotome cuts 50-1000nm plastic sections

10. Leica Ultracut UCT Ultramicrotome equipped with an EM FCS cryo stage

11. Leica CM3050 S Cryostat cuts 2-200µm frozen sections

12. LiveCell StageTop Incubation Systems

13. Eppendorf Microinjection System

14. Tousimus Autosamdri-815 Critical Point Dryer

15. Hummer 6.2 Sputter Coating System

IMAGING SOFTWARE

Volocity (PerkinElmer) for 3D rendering and analysis

Imaris (Bitplane) for 3D rendering and analysis

Huygens (SVI) for deconvolution of image stacks

Updated 12/16/10