Study Design

• Tissues
• Technology
• Network Motifs

• Raw Data
• Literature Evidence of Autoregulation
• Binding Table of Analyzed Data
• Binding Site Presence in Autoregulatory Promoters
• Supporting Material (pdf)
• ChIP Protocol (pdf)

We mapped the transcriptional regulatory circuitry for six master regulators in human hepatocytes using chromatin immunoprecipitation and high-resolution promoter microarrays. The results show that these regulators form a highly interconnected core circuitry, and reveal the local regulatory network motifs created by regulator-gene interactions. Auto-regulation was a prominent theme among these regulators. We found that hepatocyte master regulators tend to bind promoter regions combinatorially and that the number of transcription factors bound to a promoter corresponds with observed gene expression. Our studies reveal portions of the core circuitry of human hepatocytes.