Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells

Mapping Transcription Factor Occupancy in Human Embryonic Stem Cells

• Growth Conditions
• Immunohistochemical Analysis of Pluripotency Markers
• Teratoma Formation
• Human ES Cell Quality Control
• Immunohistochemical Analysis of Pluripotency Markers
• Teratoma Formation
• Embryoid Bodies (EB)
• Technology and Protocols
• Antibodies
• Chromatin Immunoprecipitations
• Microarray Design

• Download Data
• Data Analysis

Oct4, Sox2, and Nanog are thought to be central to the transcriptional regulatory hierarchy that specifies ES cell identity because of their unique expression patterns and their essential roles during early development (Avilion et al., 2003; Chambers et al., 2003; Hart et al., 2004; Lee et al., 2004; Mitsui et al., 2003; Nichols et al., 1998; Schvler et al., 1990). To further our understanding of the means by which Oct4, Sox2, and Nanog control the pluripotency and self-renewal of human ES cells, we have used genome-scale location analysis (chromatin immunoprecipitation coupled with DNA microarrays) to identify the target genes of all three regulators in vivo.

This web page provides links to further information describing the ES cell culture conditions, experimental protocols and microarray design.