Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells

Mapping Transcription Factor Occupancy in Human Embryonic Stem Cells

• Growth Conditions
• Immunohistochemical Analysis of Pluripotency Markers
• Teratoma Formation
• Human ES Cell Quality Control
• Immunohistochemical Analysis of Pluripotency Markers
• Teratoma Formation
• Embryoid Bodies (EB)
• Technology and Protocols
• Antibodies
• Chromatin Immunoprecipitations
• Microarray Design

• Download Data
• Data Analysis

H9 human ES cells were cultured as recommended by the WiCell Institute (www.wicell.org). We also tested them by immunohistochemistry for characteristic stem cell markers, their ability to generate cell types from all three germ layers upon differentiation into embryoid bodies and their ability form teratomas in immunocompromised mice.